AutoBlot Studio
Features How it works Collaborate Pricing Guides How to quantify western blots Stats & plotting guide About
Runs entirely in your browser — nothing to install

Automated Western Blot Analysis
That Actually Saves You Time

Open your browser, upload your blot, and have publication-ready figures in minutes — no installation, no IT request, no waiting. One tool for your whole lab, with unbiased, reproducible results every time.

PNG · TIFF · JPG · BMP  ·  Free tier available  ·  Desktop & iPad

Western blot DNA electrophoresis RNA gel SDS-PAGE Native PAGE
autoblot.studio/analyze
634 researchers worldwide
Minutes not hours, per experiment
Unbiased automated densitometry
All-in-one no tool-switching
No install desktop & iPad, any browser
Why AutoBlot

Built around how
researchers actually work

Five principles that shaped every design decision — from the first pixel to the last export button.

⚡
Saves time
What used to take hours — manual ROIs, background subtraction, figure assembly — is done in minutes. Automatic band detection on first click, publication figure on last.
🖱️
Easy to use
No training required. Drop in your image, click detect, export. And if you're ever unsure what to do next, just ask Blotty — the built-in AI that guides you through every tool in plain English, with zero learning curve.
🤝
Collaborative
One shared workspace for your whole lab. Supervisors, postdocs, and students all see the same blots, figures, and data — no emailing TIFFs across the hall.
🗂️
All in one
Image analysis, densitometry, statistics, lab book, and figure export — all under one roof. Stop juggling ImageJ, Prism, Excel, and Word for a single experiment.
⚖️
Unbiased
Otsu thresholding and automated background correction remove human judgement from your measurements. Every result is reproducible, traceable, and audit-ready.
Features

Every tool you need.
Nothing you don't.

One platform covers your entire workflow — from image import to publication figure — so nothing falls through the cracks between tools. Click any card to explore.

🎯 Free
Automatic band detection
Otsu thresholding and connected-component labelling finds your bands on first click, fits tight ROI boxes, and labels them left-to-right. Rename or delete any result, or draw your own boxes manually.
Ctrl TrtA TrtB TrtC TrtD TrtE TrtF
📊 Free
Unbiased densitometry
Automatically generates publication-ready bar charts with error bars from your band intensities. Background-corrected net values also export to CSV for downstream statistics in R or Prism. Ask Blotty to explain your results or suggest the right statistical test.
Ctrl A B C D E F Int.
📄 Free
Publication figures
One-click export at journal resolution. Editable labels, MW markers, and condition rows — everything composited to a single canvas. Free accounts get 1 full-resolution export per month, no watermark.
Western Blot Analysis — p-ERK / Total ERK kDa 50 37 p-ERK t-ERK Ctrl Trt A Trt B Trt C Trt D Trt E Trt F
🧬 Lab
Lab group collaboration
Share analyses with your group. Supervisor, postdoc, and student roles. Everyone sees the same blots, measurements, and figures.
Exp 42 — HER2 western · shared with 3 SL TK RB 👩‍🔬 Supervisor 🔬 Postdoc 🧪 Student SL Sarah added bands to Lane 3 2 min ago TK Tomás ran densitometry analysis 14 min ago RB Raj exported figure to PDF 1 hr ago Live sync · 3 members active
Free
Blotty — your wet lab AI copilot
Blotty isn't just a chatbot — it's context-aware across every tool. Ask it to run a statistical test, interpret your results, troubleshoot your blot, or move data between modules. It reads your actual data and acts on it directly — no clicking through menus, no learning curve.
"Run ANOVA on this" "Why are my bands smearing?" "Best way to analyse this data?" "Normalise to GAPDH" "Log this in my lab book"
Analysis suite
Normalise to housekeeper, batch-crop lanes, flag overexposed bands, export at journal spec
Stats suite
Auto-detect data type, recommend and run the right test, explain results in plain English
Lab book
Insert calculators, search past experiments, summarise entries, trigger e-signature
📈 Pro
Stats Suite
t-tests, ANOVA, dose–response curves, Kaplan–Meier survival analysis, and publication-ready graphs — all in one place.
0 25 50 75 Intensity (a.u.) Control Drug A Drug B p = 0.003 ** p = 0.021 * One-way ANOVA + Tukey HSD
📓 Pro
Scientific Lab Book
An electronic lab book built for scientists. Write structured experiment pages, embed live blot data, insert calculators with unit conversion, and e-sign records under CFR 21 Part 11 compliance — all in one place.
LaTeX equations 12 calculators Unit conversion CFR 21 Part 11 e-Signature Autosave OneDrive & cloud export
Exp 19 — p-ERK HeLa dose response 16 May 2026 · Sarah L. ✓ Signed p-ERK t-ERK IC₅₀ = 2.41 μM [95% CI: 1.9–3.1] ⊞ Molarity calculator ✦ Electronically signed — 16 May 2026, 14:22
🗜️ Pro
Multi-channel fluorescence import
Drop a .zip containing your 600 nm, 700 nm, and 800 nm TIFF files. AutoBlot composites them with a screen blend and gives you per-channel brightness, contrast, and colour controls — all non-destructive.
600
Brightness
700
Brightness
800
Brightness
🔍 Lab
Integrity Checker
Upload any published blot to detect signs of manipulation — clone detection, error level analysis, and histogram forensics in one report.
Clone detection Error level analysis Histogram forensics
Live
Trusted by the community
Growing every day — researchers across academia and industry worldwide.
634
researchers
12
countries
How it works

From scan to figure
in under 60 seconds

Watch the full workflow. Every step happens in the browser — no software to install.

autoblot.studio/analyze
1.5× speed
01

Upload your blot

Drag and drop from your scanner. PNG, TIFF, JPG — all accepted.

02

Straighten & crop

Drag to rotate. Draw a crop box, one click to apply.

03

Auto-detect bands

Hit detect. Rename each band with your sample labels.

04

Export figure & data

Publication PNG + CSV densitometry in one click.

Your workspace

Pick up exactly
where you left off

Every analysis is saved automatically. Open the dashboard and see all your recent blots, with band detections and figure settings preserved.

  • ✓
    All your images and measurements saved to your account
  • ✓
    Reopen any analysis and continue — no starting over
  • ✓
    Share specific analyses with lab group members
  • ✓
    Version history so you can see every change made
AutoBlot Studio — Dashboard
Good morning, Tom
3 analyses in progress  ·  2 shared with your group
12
Analyses
3
In progress
5
Shared
Recent analyses
p-ERK1/2 timecourse
Modified 2h ago  ·  3 blot types
Phospho
AKT / p-AKT panel
Yesterday  ·  2 blot types
Total
β-Actin loading control
3 days ago  ·  1 blot
Housekeeper
AI Copilot

Meet Blotty —
your lab AI

Blotty isn't a generic chatbot. It reads your actual data, understands your experiment, and acts on it directly — no copy-pasting, no switching tools.

📊
Runs analyses on your data
"Run ANOVA on this" → executes the test, updates the graph, shows you the result. No menus.
🔬
Troubleshoots your experiments
"Why are my bands smearing?" → protocol-specific answers based on what you're actually running.
🧠
Recommends the right approach
"Best way to analyse this?" → reads your group count, n, and data type, then recommends and asks follow-ups if needed.
📓
Works across every module
Analysis suite, stats, lab book, integrity checker — Blotty connects them all and moves data between them on request.
"Normalise to GAPDH" "What loading control for neurons?" "Log this IC50 in my lab book" "Export at Nature Cell Bio spec"
✓ Free on all plans — no extra tokens needed for data-aware commands
Blotty context-aware
What's the best way to analyse this data?
You have 3 independent groups, n=6–8. One-way ANOVA looks appropriate. Are these the same subjects measured repeatedly, or different subjects in each group?
Different subjects — just run it
Done. F(2,21) = 6.42, p = 0.007 ★★. Post-hoc: Control vs Drug A p = 0.009 ★★. Graph updated.
Collaboration

Built for
research groups

Add your supervisor, postdocs, and labmates to a shared group. Everyone sees the same analyses, figures, and raw data — no more emailing TIFFs or reconciling results from different software.

  • ✓
    Shared lab workspace — one home for all your blot data
  • ✓
    Role-based access — supervisor, postdoc, and student tiers
  • ✓
    Full audit trail — every measurement is traceable
  • ✓
    Perfect for multi-site collaborations and core facilities
PI
SR
LM
JK
Research Group
6 members  ·  24 shared analyses
TB
Lab PI
Owner
SR
Postdoctoral Researcher
Postdoc
LM
L. Morrison
PhD Student
JK
J. Kim
PhD Student
📊
p-ERK timecourse — revision 2
Shared by L. Morrison  ·  1 hour ago
New
Pricing

Start free,
upgrade when you're ready

No credit card required for the free tier. No lock-in, cancel anytime.

Pay monthly Pay annually Save 17%
Free
£0/ month

For students and researchers exploring the tool or running occasional experiments.

  • ✓Up to 5 blot images at once
  • ✓Full band detection & densitometry
  • ✓1 full-resolution export / month, no watermark
  • ✓CSV data export
  • —Unlimited images
  • —Lab collaboration
Most popular
Pro
£15/ month

Billed as £180 / year — saving £36 vs monthly

For active researchers who run regular experiments and need publication-ready figures every time.

  • ✓Unlimited blot images
  • ✓High-res unlimited exports, no watermark
  • ✓Use figures in publications
  • ✓Bulk export & .zip upload
  • ✓Scientific Lab Book
  • ✓Stats Suite access
  • ✓Export to PowerPoint
Lab
£100/ month

Billed as £1,200 / year — saving £240 vs monthly

For research groups and core facilities — up to 10 users sharing a workspace.

  • ✓Up to 10 users — everything in Pro
  • ✓Shared lab workspace & collaboration
  • ✓OneDrive & cloud upload
  • ✓Tampering detector
  • ✓Audit trail & version history
  • ✓Purchase order / invoice billing
  • ✓Dedicated support
Enterprise
Custom

Site-wide access for universities, research institutes, and hospital trusts.

  • ✓Everything in Lab
  • ✓Unlimited labs & users
  • ✓SSO / SAML integration
  • ✓Multi-lab admin console
  • ✓Role hierarchy (PI, postdoc, student)
  • ✓Annual site licence invoicing
  • ✓Dedicated onboarding & support
Starter
£0/ month

Evaluate AutoBlot for your team before committing to a paid plan.

  • ✓Up to 5 blot images at once
  • ✓Full band detection & densitometry
  • ✓1 full-resolution export / month, no watermark
  • ✓CSV data export
  • —Unlimited images
  • —Team collaboration
Most popular
Pro
£30/ month

Billed as £360 / year — saving £72 vs monthly

For scientists in industry running regular experiments with compliance and reproducibility requirements.

  • ✓Unlimited blot images
  • ✓High-res unlimited exports, no watermark
  • ✓Use figures in publications & reports
  • ✓Bulk export & .zip upload
  • ✓Scientific Lab Book
  • ✓Stats Suite access
  • ✓Export to PowerPoint
Lab
£250/ month

Billed as £3,000 / year — saving £600 vs monthly

For R&D teams, CROs, and biotech companies — up to 10 users with compliance tools.

  • ✓Up to 10 users — everything in Pro
  • ✓Shared team workspace & collaboration
  • ✓OneDrive & cloud upload
  • ✓Tampering detector
  • ✓Audit trail & version history
  • ✓21 CFR Part 11 audit support
  • ✓Dedicated account manager
Enterprise
Custom

For pharmaceutical groups, hospital networks, and multi-site research organisations.

  • ✓Everything in Lab
  • ✓Unlimited sites & users
  • ✓SSO / SAML integration
  • ✓Multi-site admin console
  • ✓Custom data retention policies
  • ✓Private cloud / on-premise option
  • ✓SLA & executive account management
Blotty · Lab Assistant