Upload any western blot image to get an instant quality and integrity report — cloned regions, compression artefacts, saturation, and histogram anomalies. Free, runs entirely in your browser, no account required.
Any western blot — published figures, submitted data, your own images
Image manipulation in western blots — splicing bands together, duplicating lanes, or selectively adjusting brightness and contrast — is one of the most common reasons figures are flagged during peer review or post-publication scrutiny. This free tool runs the same forensic checks used by journal editors and image integrity reviewers, entirely in your browser, so you can spot problems before you submit.
The checker analyses your image using four techniques:
Once you have a clean blot, you can move straight into AutoBlot Studio to detect bands, quantify intensities, normalise to a loading control, run statistics, and export a publication-ready figure.
It runs four checks on your uploaded image: error level analysis (ELA) to highlight areas that may have been edited or recompressed differently from the rest of the image, clone/copy detection to flag duplicated regions such as repeated bands or backgrounds, a pixel intensity histogram to spot saturation and unnatural gaps or spikes, and file metadata extraction to review embedded image information.
Yes, the checker is completely free and does not require an account. Your image is analysed entirely in your browser and is never uploaded to a server.
No. The results are probabilistic indicators, not proof of manipulation. Compression, scanning, cropping, and normal image processing can all produce similar signatures. Findings should be considered alongside other evidence and expert review.
The checker accepts PNG, JPG, TIFF, BMP, and WebP files.
Yes, you can export the full report as a TXT file or a PDF for your records.